PCBIS proposes a miniaturized microplate assay to combine the detection of cytotoxic and apoptotic effects of molecules of interest using a single sample.
This assay is relevant for the characterization of cytotoxicity of molecules or plant extracts.
The CellTiter-Blue assay (PROMEGA) allows the quantification of cell viability by measuring the production of resorufin after resazurin reduction by viable cells.
The fluorescent signal (ex 560 nm / em 590 nm) produced by resorufin is proportional to the metabolic activity of the cells. Since it does not lyse cells, this assay can be combined with other assays to study several cellular parameters.
The Apo-One Homogeneous Caspase 3/7 Assay (PROMEGA) allows the quantification of Caspase 3/7 activity characterizing the apoptotic process.
The assay is based on the cleavage by caspases 3/7 of the non-fluorescent substrate Z-DVED-R110 into fluorescent Rhodamine 110. The fluorescent signal (ex 499 nm /em 521 nm) produced by Rhodamine 110 is proportional to the cell apoptosis
| Format | 96 wells | ||
| Cellular models | Several cell lines available | ||
| Reference molecules | Staurosporine / Ionomycine | ||
| Incubation | 24H at 37°C under 5% CO2 possibility to adapt the incubation time to the biological model | ||
| Detection | Fluorescence measurement | ||
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Recommendation :
All models can be adapted to your request.