Rapid and Highly Selective Fluorescent Labeling of Peptides via a Thia-Diels-Alder Cycloaddition

we describe a catalyst-free thia-Diels–Alder cycloaddition for the chemoselective labeling of fully deprotected phosphonodithioester–peptides in solution with fluorophores functionalized with an exocyclic diene.

doi: 10.1021/acs.bioconjchem.2c00500