Pathophysiological investigations

Measuring the activity of a drug candidate in pathophysiology requires the exploration of phenotypes (lung function, inflammation and remodeling in tissues, collection of blood, cells and tissues...). We have developed standardized techniques for exploring inflammation.

Effect of a drug candidate on respiratory function

Respiratory function can be explored in all models of respiratory disease to follow changes in frequency, flow, pressure… for longitudinal studies or in response to a pharmacological compound such as methacholine to highlight airway hyperresponsiveness in asthma.

  • Whole body plethysmography (EMKA technologies) : designed to study airway response in awake and unrestrained mice. It is a non-invasive system that allows for longitudinal study.
  • Forced oscillation technique (Flexivent, Scireq) : designed to measure the resistance, elastance and dynamic compliance of the airways in anesthetized animals (terminal).

Bronchoalveolar lavage

In mouse models, bronchoalveolar lavage allows sampling of inflammatory cell populations. This technique is essential for studying cell influx in models of lung diseases such as asthma and COPD. Cell counting can be combined with methods such as flow cytometry, ELISA, immunocytochemistry, immunohistochemistry, PCR or HPLC to assess inflammatory components such as cytokines, growth factors, analysts and receivers.

Flow cytometry

We have developed an easy-to-use, easy-to-customize, one-step, multicolor flow cytometry method for performing differential cell counting. This method identifies bronchoalveolar lavage cells by a one-step simultaneous immunostaining procedure using antibodies to identify T cells, B cells, neutrophils, eosinophils and macrophages. An important advantage of this flow cytometry protocol is the ability to customize it by adding antibodies to study receptor expression at the leukocyte cell level and identify inflammatory cell subclasses as needed.

PCBIS and you

All explorations can be adapted to your request.