The hemolysis test assess the hemolytic power of a compound, i.e. its ability to destroy the red blood cells.

Protocol

MatrixMouse or human blood
Test concentrations

200, 100, 20, 6, 2, 0.6, 0.2 et 0.06 μM.

Test temperature37°C
Incubation time45min
ControlTriton X-100
Analysis methodAbsorbence at 535 nm
Data deliveryDose-response curve, IC50
Replicate2