Shake-flask method allows to determine thermodynamic solubility.

It’s not always necessary to have such an information, in some cases, an apparent solubility is enough.

Drug screenings are usually performed from chemical libraries prepared in DMSO stock solution.

It’s possible to determine an apparent solubility from these stock solutions by preparing a dilution in an aqueous buffer.


MatrixPBS pH 7.4
Test températureRoom temperature
Incubation time24h
ControlsAcetaminophen, Tamoxifen, Rifampicin, Haloperidol
Analysis methodHPLC-UV
Data deliverySolubility in µM



For another buffer or different incubation temperature, please inquire.

For another solubility threshold than 200 µM, please inquire. DMSO concentration should be 2% maximum.