Metabolism corresponds to the transformation of compounds in the body by enzymatic means.

The main organ involved in this process is the liver.

Microsomes are a subcellular fraction of liver homogenate. They contain the enzymes bound to the membrane of the endoplasmic reticulum like cytochromes P450.

The use of microsomes is suitable for a study with a high throughput of analyzes (large number of compounds).


MatrixPhosphate buffer, mouse or human liver microsome (0,5mg/mL)
Test concentration1µM
Test temperature37°C
Time points0, 2, 10, 20, 40 and 60 min
ControlsTestosterone and dextromethorphan (mouse) and verapamil (human)
Analysis methodLCMS-MS
Data deliveryHalf-life




Time points can be customized