Metabolic stability on hepatocytes

Metabolism corresponds to the transformation of compounds in the body by enzymatic means.

Hepatocytes possess the major enzymes allowing the metabolism of xenobiotics.

They allow the simultaneous study of the main biotransformation reactions carried out in the liver: hydrolysis, reduction, oxidation and conjugation.

This test consists of measuring both the disappearance of the starting molecules and the appearance of their metabolites.

Hepatocytes of different species are available: human, mouse, rat, dog, monkey.


MatrixIncubation medium, human or animal hepatocytes (2.106 cells/mL)
Test concentration1 and 10 µM
Test conditionsIncubator at 37°C, 5% CO2
Time points0, 5, 15, 30, 60 and 120 min
ControlsTestosterone and Dextrometophan
Analysis methodLCMS-MS
Data deliveryHalf-life, identification of metabolites




Time points can be custumized.