Blood and plasma contain enzymes that can hydrolyze some labile groups such as esters or amides.

It is important to determine plasma stability of a new molecule because compounds rapidly degraded in plasma generally have low activity in vivo.

Conversely, in the case of "prodrugs", the hydrolysis of labile groups makes it possible to increase the pharmacological activity.


MatrixMouse, rat or human plasma
Test concentration1µM
Test temperature37°C
Time points0, 4, 20, 40, 80 and 120min
Analysis methodLCMS-MS
Data deliveryHalf life



Time points can be personalized.

The plasma stability of a compound can vary greatly from one species to another.

Stability can be measured in whole blood as well.