Histamine is a mediator involved in inflammatory and allergic phenomena with immediate manifestation. It is massively secreted by mast cells and basophilic polynuclei after contact with an allergen.
The determination of histamine is therefore a relevant test for the identification of potential allergens.
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Two compatible fluorophores, donor (europium cryptate) and acceptor (d2), are bound to an anti-histamine antibody and to histamine respectively.
Histamine from the sample competes with histamine linked to the acceptor fluorophore for the binding to the antibody, preventing the time-resolved energy transfer (TR-FRET) between the 2 fluorophores.
| TREATMENT | |||
|---|---|---|---|
| Format | 96 wells | ||
| Cellular model | Human Mast Cells-1 | ||
| Stimulation | A23187 / PMA | ||
| Reference molecule | Quercetol | ||
| Incubation | 18H at 37°C under 5% CO2 | ||
| Revelation | HTRF ASSAY | ||
| Format | 384 wells | ||
| Detection | Detection Excitation wavelength : 337 nm Emission wavelength 1: 615 nm (donor fluorophore emission) Emission wavelength 2: 665 nm (acceptor fluorophore emission) | ||
Notes :
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