AlphaScreen™ (Amplified Luminescent Proximity Homogeneous Assay), developed by PERKIN ELMER, is bead-based assay technology used to study biomolecular interactions in a microplate format.
This assay is homogeneous and requires only small amounts of protein.
Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm
If the donor bead is close to the acceptor bead (< 200 nm), energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm
Beads can be coupled to many molecules allowing the capture of proteins fused to specific sequences.
AlphaScreen signal is proportional to the protein-protein interaction.
| Protein-protein interaction | |||
|---|---|---|---|
| Format | 384 wells | ||
| Protein 1 + Protein 2 +/- inhibitor Reaction conditions to be defined | |||
| Revelation | Donor/acceptor beads mix Conditions to be defined | ||
| Detection | AlphaScreeen measurement | ||
Notes :
Allow 50 µL at 10 mM or 3 mg of sample to test 5 concentrations (3 points/concentration)
All models can be adapted to your request.