Introduction
The Label Free biochemical test, based on Corning Epic® technology, allows to measure the binding of a ligand on a protein target without any label.
Principle
The target protein is immobilized onto the amine-coupling surface of label-free biochemical microplate biosensor.
For interactions that are more difficult to detect, a spacer can be added to make the protein more accessible.
The bottom of the plate is illuminated by a broadband light. A reference area prevents non-specific target immobilization.
Biochemical binding events cause index of refraction changes, resulting in a picometer wavelength shift.
Protocol
To be adapted according to the test
| Format | 384-well | ||
| Plates | Pre-activated or operator activated plates | ||
| Immobilization of the target | 1H at 21°C | ||
| Incubation | O/N at 4°C | ||
| Washing | PBS wash | ||
| Incubation | 4H at 21°C | ||
| Baseline reading | |||
| Treatment | conditions to be defined | ||
| Final reading | |||
Notes :
- Plan for 5 mg of protein and 1 mg of ligand
Measurement of biotin binding to streptavidin
All models can be adapted to your request.