Monitoring the efficiency of a transfection

Introduction / Principle

Monitoring the efficiency of a transfection can be performed by following the increase of fluorescence from of a fluorescent protein such as GFP which is transfected at the same time of the gene of interest.
Fluorescence monitoring can be performed with an automated cell imaging system "IncuCyte" (SARTORIUS).


FormatsFlasks or 6 to 96 wells culture plates
Cellular modelAll types of adherent or suspension cells
IncubationTo be adapted according to the biological model
DetectionIncucyte® S3 (SARTORIUS) - Fluorescence

The fluorescence intensity of GFP expressed over time is recorded by the Incucyte.