Introduction / Principle
A 3D cellular model, such as spheroids, offers an intermediate level of complexity between monolayer culture and the use of animal models, by reproducing in vitro the organization of a micro-tumor. This model has been shown to be more predictive of treatment response than monolayer culture models.
Thus, PCBIS proposes a miniaturized microplate assay to perform cell proliferation kinetics under conventional culture conditions in a controlled atmosphere by quantifying the area of spheroids using the Incucyte S3 (SARTORIUS).
