Introduction
Dynamic light scattering (DLS) is commonly used in particle size analysis as an indicator of solution homogeneity or aggregate formation.
This technology can be used to find the best conditions for protein crystallization.
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Dynamic light scattering (DLS) is commonly used in particle size analysis as an indicator of solution homogeneity or aggregate formation.
This technology can be used to find the best conditions for protein crystallization.
Particle size from 1 nm to 1 µm is determined by measuring random variations in the intensity of light scattered by a suspension or emulsion.
www.wyatt.com/solutions/techniques/dynamic-light-scattering-nanoparticle-size.html
Small particles in suspension are subjected to a random thermal motion called Brownian motion. This motion is correlated to 1) the hydrodynamic diameter of the particles, which corresponds to the size of a sphere that would scatter light in the same way, and 2) the transverse scattering coefficient which is measured by dynamic light scattering. Viscosity and temperature also come into play.
Format | 384 wells | ||
Detection | Dynapro Plate Reader® Wyatt Technology 10 acquisitions / well 5 seconds / acquisition |
Volume : 60 µL
Concentrations : 0,015 to 2 mg/mL