Screening Department
Inflammation
Quantification of cytokine secretion by mononuclear cells
Assay of nitric oxide secretion by murine macrophages
Determination of histamine secretion by human mast cells
Enzymatic activity
Molecular interactions
Measurement of macromolecules interactions by AlphaScreen (PERKIN ELMER)
Search for interaction between a soluble protein and a ligand by fluorescence polarization measurement
Measurement of molecular interactions using Epic Label Free technology
Membrane receptor - ligand binding
Measurement of receptor-ligand binding by FRET
Measurement of receptor-ligand binding by HTRF
Measurement of receptor-ligand binding in whole cells without labeling
Functional assays on GPCR
Intracellular IP1 measurement
Intracellular calcium flux measurement
Intracellular cAMP measurement
Cell growth and cytotoxicity
Monitoring the growth of a bacterial strain
Monitoring cell line growth
Monitoring the growth of single spheroids
Evaluation of the cytotoxic effects of a compound on cell lines
Evaluation of the cytotoxic effects of compounds on single spheroids
Detection of cell apoptosis
Scratch Wound Healing assay
Evaluation of the angiogenesis process by endothelial cells
Efficiency of a transfection
Monitoring the efficiency of a transfection
Intestinal barrier permeability/integrity
Evaluation of the absorption of a compound through the intestinal barrier
Evaluation of the effect of compounds on the integrity of the intestinal barrier
Aggregation, particle size (DLS)
Particle size measurement by Dynamic Light Scattering in 384-microplate