Our research and development projects aim to explore new approaches aimed on the one hand at facilitating the study of PMs in solution and, on the other hand, at promoting more generally our tools and our know-how in bioproduction. These projects, most of which are carried out in a collaborative framework, are currently structured around the following axes:
• Development / optimization of the bioproduction capacities of the P. pastoris system
- Evaluation of new culture / induction conditions and formats
- Evolution of producer strains by genetic engineering (CRISPR-Cas9 gene editing)
- Development of expression tools and strategies (inducible promoters, fusion sequences, gene co-expression, etc.)
- Evaluation of the system's performance for the production of complex proteins (membrane oligomers, multiprotein complexes, pseudo-viral particles, etc.)
• Methodologies for preparing MPs in different membrano-mimetic environments
- Extraction of MPs from different sources (membrane fractions, protoplasts)
- Evaluation of various surfactants (detergents, amphiphilic polymers)
- Evaluation of different membrano-mimetic environments (detergent micelles, bicelles, lipid nanoparticles, proteoliposomes).
• Methodologies and tools for the production and isotopic labeling of MPs for NMR analyses
- Development of uniform labeling methods
- Development of selective labeling tools and methods
- Evaluation of sample preparation conditions for solid-state NMR
• Methodologies dedicated to the development of immunologics targeting MPs
- Evaluation of immunization and antibody selection strategies adapted to PMs
- Evaluation and characterization of different immunologics (conventional antibodies, camelid antibodies, antibody fragments, etc.)
