The effect of compounds on the integrity of the intestinal barrier can be assessed by measuring the change in resistance of a monolayer of Caco-2 cells after addition of the compound.
The Caco-2 cell line (derived from human colorectal carcinoma) is the model used in vitro to characterise the effects of compounds on the intestinal barrier.
When grown on semi-permeable membranes, Caco-2 cells differentiate to form a highly functionalized epithelial barrier with remarkable morphological and biochemical similarity to the intestinal epithelium.
Caco-2 cells are grown on a membrane placed between two compartments.
The compounds are added to the apical side of the cell monolayer.
The effect of the compounds is assessed by measuring the trans-epithelial electrical resistance of the cell monolayer.
| Format | 24-well format | ||
| Cell model | Caco-2 (ATCC HTB-37) differentiated | ||
| Reference molecules | IFNɣ + TNFα | ||
| Treatment | 2 concentrations - 3 points | ||
| Incubation | 72H à 37°C under 5% CO2 | ||
| Analysis | measurement of cell resistance (TEER) over time | ||
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